Natural transformation is when bacteria take up naked bacterial chromosomal DNA in their environment (usually from cell lysis). A cell "lysate" is what remains of bacterial genes when the bacteria is dead (can be extracted from bacteria, as shown here). The SHiN bugs all can undergo transformation. You know it is transformation even without knowing which bugs can do so because it doesn't take up the DNA when DNase is added (it kills any free environmental DNA in the lysate)
topgunberjust wondering why is plasmid transfer not a good option???+2
m0niaguiTransduction requires the presence of a bacteriophage virus. Plasmid transfer requires two different live bacteria, point mutations will not occur across colonies so uniformly and neither will strand mispairing.+1
shakakaka@topgunber I think DNase wouldn't stop the process in case of plasmids+4
topgunberyou're right in that DNAse wouldn't be able plasmids in living cells because they are inside the bacteria (same with their nuclear dna). Since living cells use sex pilli to transfer plasmids yes, DNAse wouldn't stop plasmid transfer. Key there is they had to be living. I do think a dnase can break down a plasmid in extracellular solution though (its just another piece of dna).+1
l0ud_minorityKey is heat-killed here and if you remember Griffith's Experiment it relates to transformation+
submitted by โitsalwayslupus(48)
Natural transformation is when bacteria take up naked bacterial chromosomal DNA in their environment (usually from cell lysis). A cell "lysate" is what remains of bacterial genes when the bacteria is dead (can be extracted from bacteria, as shown here). The SHiN bugs all can undergo transformation. You know it is transformation even without knowing which bugs can do so because it doesn't take up the DNA when DNase is added (it kills any free environmental DNA in the lysate)