heT ewraeLinev ukrB tlPo is lmayni tdprretniee yb tis X and Y tsTeri nhce.pet iqonuste sttesa hatt vgingi 6B rsiceesan het caivttiy to onlarm v,elesl wihhc emsan teh acivitty udsloh be the saem sa hte a,rlcH.nm eneo theri Y tenietrcp doluhs be teh ,msae so ew era eetwneb eshccio B dna .C A adn D do ton haev hte smae Y pecnittre as iganrhHmr.ole tacntnieoonsrc of B6 ueacsd tavciyit to ecmobe almrno, and os amVx lwil tno eb ancghgni dna ew nca canelc A nad A.D hsa a wreol xVam nad D sha a umch ihregh xa.heV mT rfeidnefce nwebeet B dan C is ni rthie Km. novgiM ot the left on eth X sxai skema het Km lorwe nad tlels ouy tath nfiyatif si ghirheos ouy owdul otn eend rome 6B. tuB in oru cesa yatiinff fo teh eyemnz rof 6B si aerlly o,wl iwchh is wyh ew eden a tno remo 6.B
In rsmu,aym ew atwn het esma xmaV dan a hiehrg KWem . ntaw eth "nol"ram yvtctaii ea(sm amvx sa )lanmro adn ew eedn rhhieg otumnsa of B6 rfo ccussse ayii fsntof fo eth zeyenm orf 6B si ylbbaorp yerv lo.w
cheoCi B hsa a -mK)1/( vaule lrecso ot 0 ihcwh masne Km si woelr and atyiffin fo hte neyezm rof sti ertsabtsu is srpeu soTw. ihl mseak senes as nvigig rhgihe soamntu of teh toipvi""cmeet teaubtssr 6B si lhi.ngep
enidNge r"ihgeh enrttainnsoocc" fo hte B6 ofr znemey ityvacit is oathrne awy fo ngsayi Km is ihergh esicn meor is eqriredu rof 12/ xavm ycitva.ti nIsrdecae Km saveul uetlrs in m1K/ eigbn marlsle e(lscro ot 0 no het x,-)sxai chwhi is daoreedtstmn ni rwsane eccohi B.
A "moa"lnr neyemz iictavyt in eht reecsenp of ihhger B6 ntrnaocntieocs naems hatt xmVa si otn a,ngcihgn utb mK .si
eSmo of eesth plannotaexis leylar giodn hte os.tm If the yenzem si lbea to sareeicn to nalrom caiiyvtt thwi a hhgeri leelv fo hte est,trbusa ttah aesmn e’wer ni a ceivmioeptt birhnioit intuioa.ts Cvmeeittiop yawals coresss on eht rpg,ah os ew cna mtaelinei A nad D.
Now ew yzleaan m.K eW twan llmsa sKm’ orf opettn m.yesnez zieS sdoe tno lasway rmetta 😉. cetpxE in stih seac we on’dt watn htat pe,oytnc we wtna to kool rfo a l/le1r(ay gib ),Km au(vel thta si lsecor to zr)e,o bc we watn a wake bgi mK to eb aelb to wroerepvo het pcomtievtei n.ibrihiot npromgiaC hte ignneiram 2 cihc,seo B sha a mK lueav lorcse ot ezor so ’sehrte oyru rewns.a
For isth esioq,tnu I saw kihgtnni taht heyt sdkea oyu rpoaemc to a “rmanlo” srnp,oe os eth Vxma dlow’ntu cgaenh inces tyeh ttsea ahtt hsi tycvtiia usrrent ot a morlan vllee. lnyO hte ixxas- ldouw ceisn ti olduw be at esiardcen itrcanoestnnoc in ar.mnscopio
So i nudof hist otnuiesq ri.ou.cl.dsiu het osnpaaenixlt ivdrdepo did nto llaeyr creal ti pu for e,m but i nthki i aseurdndtn it own e(lepas croertc em if mi' .r)ngow
rsitF nda sfootrme, m'I ont vene ersu if tish is lryut a vanl-eieburrweek t,opl ncise het -sixaX si ton ./K1m
Btu, ermo realy,genl I irdet ot dersudantn thaw pneadhpe ta thbo etxesemr of ][PLP dan iotelc.yV
PPA:skL :syulfero ehwer on the rhapg is PL[]P shei?thg leW,l fi eht X-sixa is elqua ot /,PL1P LPP oludw eb ta sit hsihtge at hte veual setolcs to 0 no the -ixxa.s n:khi(t thwa is 1 dvddiei yb a ?olibnli EVYR COELS OT ZE.R)O siTh wodlu eocsrponrd ot a ntopi oangl teh sixay- rfo hte esnzyme ivtatiy.c nI heotr ,owdsr PPL si at ist htegsih etionarncncto at teh gprsah it-rp.eecntY
ebRmreem athw we also :sdia nweh the etduamt yenmze is drouan otsl of P,LP ti ailyacbls innsotufc keli a lmn .zemney hTis aem,sn ahtt eht maVx of eht aemdtut enyezm is uaieevqntl ot the mVax of eth ramlon .enemyz ohreTe,ref ehT x1m/Va of obht neysemz era evelit.na..qu ta eht !yeencirtTs tih-p is gbi! If uoy etg tsih f,ra ehnt you acn tlmiiaeen )(A adn ,(D) iesnc shtee ahrpgs veha efnedftir srncYepe-tit eif(rtfdne xVem-sa) rfo ihret evsiceeptr .yemzens
ongviM n...o
loectVyi )V(: e hnW V si at its tigehsh (.ei. mV)ax, eth vealu of /1V doulw eb ta sti .stlewo Ainag:( kitnh of it acar:tlcylip hwat si 1 vidided by 1 oinilbl? )!0~ hr,feToere teh vluea fo 1/V si ta tis stwleo wenh hte a-iysx si aeuql to 0. rTeeeo,rhf Teh amVx fo iths rcscou wenh the axsiy- si celso to o.ez.r. sHree eht tghni ..hto htese enmszey eureirq ifneerfdt llvsee of LPP ot tepoare at aV.xm- ee,rRbemm teh a-mxV fo eht TAETMUD eynzme lliw hanpep at a uchm RHGHEI ]PP[.L Or nsvcrl,eoye eth mxaV- of teh LMNROA emnzye lwli apphen at a uhcm EOLWR [L.P..P]
be arawe thta hte xsx-ia rof tihs qetoinsu si ton ""mK Bvit(6 is nto the 'tutbasstathre i6B.V]1[/t to p,myisfi ucseabe the xamV si noagn tasy the ms,ea uyo jstu ende to ansercei ][6Bvit ot gte het sema amxV aka( histf to rhitg no hte ais.x)-x D 'ont eb loeodf nrduao yb the osntqeiu i.ewrrt
bNydoo ash nesadwer tihs sutqnioe throguh het snel of a nelgianr citjeoebv euitq ety so ser'eh my 2 netc:s Km si AEECSNDIR bc ihst nitapet hsa an emyzne whit an eolvalr LWOER aftiyfin acn( eb cerdoterc to lmraon rtefa na easricne ni aesrtustb) wihch is ahreccctsiirta fo COPIVIETMET inbhon,iiit chhiw is hwy eth line of eht .tp si eno fo iecovtimpte boinhiitin no a BL tlop
eTh ntqsiuoe is iyclbaals tesingt pu a tatosnuii miarsil to atht fo a ptmetiicevo niot.ibihr hyTe ysa ttah ighehr rsonianntcecto fo dxoayirpl taeshhppo necssiare the ttyviaci fo eht zmyene ot nrmlao vleels. i,ymrlSail ehwn you heav a mvtetoipice nioiitbrh adn sineerca teh treoncntciona fo ets,subart teh eymnze acn iachvee eht aesm Vmxa as if owthitu eht rboitihin.
rebmReme 1) cipeieomvtt hobirnisti ~ seinl rsosC at syc-i2ten tr)e ovinpectmNio-te thoiirsbni ~ no einl csros
gonL wnsare hdea,a utb brae hwti em.
:THNI v osolk dikn fo lkie ,y hrwsaee k oslok orem eikl x.
eyiectnr-tp = ax/mV1
xecerntp-ti = 1m/K
Noet ahtt a,xVm as a maereus fo nfe,arrcmpeo nca eb talerde hturhgo ynma .sgtnhi ,nlwihMaee Km si a est rshceirictaact fo teh zeyenm, nad ncnato eb rtelade.
In htsi p,eamlxe hte mzeney eanrfmorcep (Vx)am si adrsencie yb iesnanircg eht ivtnaim otaofrcc so thta it reehacs a onmra"l" .iitcaytv H,vroeew teh eneymz si llist nnlietyhre yshtit edu to a aongineltc fee,dct so teh mK ystas het .amse
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$279$49Y!GSU oYu rllyea veah ot loko at hte axis .beslla heT seqnouit ayss the .tp's tnecnysioahti nasteysh ivtiacyt )ix(sya- is LOMNRA htiw na CEADESRIN auomtn fo yplxradoi htspeoaph sx.-xi)a( xs-Yai is eth smae as A,MLNRO si-xxa sshfit ot het irtgh ngsiwoh SCIEANRDE ylxoiradp hpeaophst
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