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NBME 23 Answers

nbme23/Block 1/Question#16

Physical analysis of the isolated genomic DNA from ...

Methylase

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 +3  upvote downvote
submitted by thomasalterman(76),

Methylase methylates DNA, making the DNA resistant to restriction endonucleases

*https://d2jmvrsizmvf4x.cloudfront.net/7Pf4zZ3TuGwLTRKvUGYL_methylatedrestriction.jpg





 +1  upvote downvote
submitted by stinkysulfaeggs(12),

Me reading this question stem: .....do you mean which of the following ENZYMES?





 +0  upvote downvote
submitted by consistentwrongdoer3(6),

Why does methylation cause loss of resistance to GATC restriction endonuclease? Does this have to do with methylation of U to T?

methylased  GATC related to methylase --> https://en.wikipedia.org/wiki/Dam_methylase +2  
sympathetikey  Dam methylase, alright +2  
yogi  https://www.neb.com/tools-and-resources/usage-guidelines/dam-and-dcm-methylases-of-e-coli +2  




one does not simply “walk into morder”