and we’re back! with BRAND SPANKIN' NEW tangents!
welcome redditors!to snoo-finity ... and beyond!
home login register / contact us

NBME 23 Answers

nbme23/Block 1/Question#16

Physical analysis of the isolated genomic DNA from ...

Methylase

Login to comment/vote.

 +3  upvote downvote
submitted by thomasalterman(72),

Methylase methylates DNA, making the DNA resistant to restriction endonucleases

*https://d2jmvrsizmvf4x.cloudfront.net/7Pf4zZ3TuGwLTRKvUGYL_methylatedrestriction.jpg





 +1  upvote downvote
submitted by stinkysulfaeggs(9),

Me reading this question stem: .....do you mean which of the following ENZYMES?





 +0  upvote downvote
submitted by consistentwrongdoer3(6),

Why does methylation cause loss of resistance to GATC restriction endonuclease? Does this have to do with methylation of U to T?

methylased  GATC related to methylase --> https://en.wikipedia.org/wiki/Dam_methylase +2  
sympathetikey  Dam methylase, alright +2  
yogi  https://www.neb.com/tools-and-resources/usage-guidelines/dam-and-dcm-methylases-of-e-coli +2  




for every thousand thrashing at the branches of evil
there is one hacking at root